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Intelligent Enterprise Solutions,
Inc. |
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Immuno-qPCR Hands-on Course
552
Del Rey Avenue, Sunnyvale, CA
94085
This course explains the basic knowledge for protein detection through Immuno-qPCR. The course consists of a theoretical part and a practical part where participants get to do Immuno-qPCR experiments by themselves under experienced supervision. Our four day course offers researchers, Students and professionals an explanation, teaches how to use and interpret real-time PCR gene expression data, and classify samples based on real-time PCR expression profiling. This course offers a unique opportunity to the participants in working with equipment available in the field. Scientists, Professionals and students are eligible to attend. This is also an excellent opportunity to network with industry experts. Enrollment limited.
This training program will be conducted in collaboration with TATAA Biocenter, Sweden. TATAA Biocenter offers hands-on training on regular basis in Belgium, Czech Republic, England, France, Germany, Italy, Norway, Sweden, Canada and US. Further TATAA Biocenter supports qPCR courses organized by the European Molecular Biology Organization, the Federation of European Biochemical Societies, UNESCO, Howard Hughes Medical Institute, the Swedish Development Cooperation Agency and Pittcon. TATAA obtained support from the companies like Agilent, Bio-Rad, Biosearch, Corbett Research, Eppendorf, Fuji, Invitrogene, Metabion, Roche, Stratagene, and Tecne.TATAA Biocenter trained about 1000 scientists through their training programs.
Day 1- Introduction to qPCR, Assay Design and Optimization
09.00-10.30: Basic PCR and qPCR theory and applications
-Amplification and detection
-Detection chemistries
-Selected applications
10.45-11.45: qPCR experiment
-Practical considerations when preparing PCR reactions
-Demonstration of qPCR software
11.45-12.30: Data analysis
-How does qPCR software process the data?
-How are standard curves used and created?
-How are melt curves used?
-Principle of quantification using standard curves
-Principle of relative quantification
12.30-13.30: Lunch
13.30-14.00: Analysis of performed qPCR experiment
14.00-15.00: Optimization experiment
-Optimization of Mg and primer concentration
-Programming qPCR machines
15.00-15.45: Primer and probe design and considerations
-What does primer design affect?
-What are primer dimers?
-How do we minimize formation of primer dimers?
-Design of Molecular Beacons and TaqMan probes
16.00-16.45: Optimization of qPCR protocols
-What parameters can/should be optimized?
-An optimization strategy
-Multiplexing possibilities and problems
16.45-17.15: Analysis of performed optimization experiments
17.15-17.30: Discussion and Q&A
Day 2- Quantification and Normalization
09.00-09.50: qPCR quantification strategies
-Standard curves
-Relative quantification
-How to compensate for inhibition in biological samples
09.50-10.15: Normalization of qPCR data
-What levels of normalization can be used?
-How to choose a good reference gene?
10.30-11.45: Experiment comparing different quantification strategies
-Relative and standard curve quantification
11.45-12.45: Lunch
12.45-13.45: Absolute Quantification strategies
-What is a suitable standard?
-Use of internal controls
14.00-15.00: Quantification calculation examples
-What effect will efficiency have on quantification?
-Quantification methods, and equations
15.00-16.15: Analysis of experimental data
-Differences in quantifications strategies
-Pros and cons of different methods
16.15-17.00: Discussion and Q&A
Day 3- Sample Preparation and Reverse Transcription
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9.00-10.00: Principle of RNA and DNA extraction
-How it works
-Available methods and products suitable for qPCR
-Practical considerations
-Sampling
10.15-11.15: Principle of RT and different RT priming strategies
-Pros and cons of different methods
11.15-12.00: Reverse transcription experiment using different priming methods
-Oligo(dt)
-Random Hexamers
-Gene specific primers
12.00-13.00: Lunch
13.00-13.45: qPCR experiment evaluating RT using the generated cDNA
-Is there a best RT priming method?
13.45-14.15: SNP detection
14.30-15.30: Statistical tools for data analysis
15.30-16.15: Analysis of experimental data
-Which priming method is best?
-How should experiments be planned to take RT priming into consideration?
16.15-17.00: Discussion and Q&A
Day 4- Immuno-QPCR
Theoretical background of immuno-qPCR
Technology description
How to design your own immuno-qPCR assay
How to optimize the parameters for assay development
How to analyze immuno-qPCR data
A general troubleshooting for typical errors
Application examples
Registration Fee(Lunch and refreshments are included):
Early(ends on Nov. 30th 06): Professionals/Corporate- $1400, Academic/Non Profit- $1325 and Student- $1300
Late(from Dec.01st 06): Professionals/Corporate- $1600, Academic/Non Profit- $1400 and Student - $1350
Payment Options:
For online registration using credit card please click "Register" button on the top or bottom of this page. Payments by checks need to be sent before deadlines to: Intelligent Enterprise Solutiions, Inc, 552 Del Rey Avenue, Sunnyvale, CA 94085. Please write the check to "Intelligent Enterprise Solutions, Inc."
Hotels
info. and Help:
Information on hotels and help will
be provided upon request.
Cancellation Policy:
A fee of
$100 will be applied for cancellations requested before Nov. 30th and 15% on
registration fee later. No refund if claimed after 11th of Dec. 06. For
cancellations please contact us by email at: qpcr@iesols.com. Cancellation of
the training program is possible due to unavoidable circumstances.
Directions to venue: Click here
Supporting Companies:
Contact:
552 Del Rey Avenue, Sunnyvale, CA 94085
Email: qpcr@iesols.com/cj@iesols.com
PH: 408-416-3812
www.iesols.com